※ 引述《[email protected] (希望這世界可以單純一點 )》之銘言： > 最近在看一篇paper > 有提到PCR的技術 > 有些部分不是很清楚 > 希望板上的各位大大 能幫忙解答 謝謝 > 這篇paper所用的primers 序列為 > 338f(5'-ACT CCT ACG GGA GGC AGC-3') 與 > 907 revers(5'-CCG TCA ATT CMT TTR AGT TT-3') > were used, with M being a 1:1 mixture of A and C. > 其中907 primer的序列中M與R是代表什麼意思? Standard IUB/IUPAC nucleic acid codes Code of Nucleic Acid(s) A Adenine C Cytosine G Guanine T Thymine U Uracil M A or C (amino) R A or G (purine) W A or T (weak) S C or G (strong) Y C or T (pyrimidine) K G or T (keto) V A or C or G H A or C or T D A or G or T B C or G or T N A or G or C or T (any) Standard IUB/IUPAC amino acid codes One-letter code / Amino acid / Three-letter code A Alanine Ala B Aspartic acid or Asparagine Asx C Cysteine Cys D Aspartic acid Asp E Glutamic acid Glu F Phenylalanine Phe G Glycine Gly H Histidine His I Isoleucine Ile K Lysine Lys L Leucine Leu M Methionine Met N Asparagine Asn P Proline Pro Q Glutamine Gln R Arginine Arg S Serine Ser T Threonine Thr V Valine Val W Tryptophan Trp X Unknown Xxx Y Tyrosine Tyr Z Glutamic acid or Glutamine Glx -- ┌─────◆ＫＫＣＩＴＹ◆─────┐ ＫＫ免/費/撥/接 ◤ │ bbs.kkcity.com.tw │▏電話(1)：449◤1999 電話(2)：4058-6000 └──《From:143.48.8.154 》──┘▏帳號：kkcity 密碼：kkcity --