作者nikkori (calibrated)
看板BioChem_91
標題Re: [心得] 張靜仁老師的paper
時間Wed Nov 27 19:01:09 2002
※ 引述《lptcarol (paper何其多)》之銘言：
: 誰知道那個染劑的作用??有啥相關paper可看嗎??
Stain Technol 1984 Jan;59(1):7-12 Related Articles, Links
Identification in histological sections of species origin of cells from
mouse, rat and human.
Cunha GR, Vanderslice KD.
A simple method for identifying the species of origin of mammalian cells
in tissue sections using Hoechst dye #33258 is described. This rapid
procedure involves staining fresh frozen or formalin fixed paraffin
sections with 4 micrograms/ml of Hoechst #33258 for one minute at room
temperature; following one to five minutes of washing in running tap
water, the slides are coverslipped using McIlvaine's buffer (pH 5.5)
and sealed with clear lacquer. Sections examined by fluorescence microscopy
indicate that mouse cells contain several small discrete intranuclear
fluorescent bodies, which are absent in cells from either rat or human.
This simple technique should be useful in studying developmental phenomena
in chimeric organs.
PMID: 6206625 [PubMed - indexed for MEDLINE]
不過中研院生圖中的那本找不到
架上 附近 書車 影印區都沒有 =.=
: ※ 引述《cationhsu (想找個伴(B))》之銘言：
: : 我也是...
: : 查來查去就這些啊....
: : 搞不懂....
: : 還有...那個染劑....
: : 我是有在molecular cloning中有看到...
: : 但他都沒說在不同生物中有差別的原因啊？！
: : 有沒有人知道哪有相關資訊啊...
: : 提供一下吧....
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