※ 引述《lgirl (单身日记)》之铭言： : ※ 引述《Filippo (小波)》之铭言： : : real time~ : : http://www.vu-wien.ac.at/i123/VIROKURS/RT-PCRkurs.html : : 一半德文一半英文 : The method measures PCR product accumulation through a dual-labeled : fluorogenic probe (i.e., TaqMan Probe). This method provides very : accurate and reproducible quantitation of gene copies. Unlike other : quantitative PCR methods, real-time PCR does not require post-PCR : sample handling, preventing potential PCR product carry-over contamination : and resulting in much faster and higher throughput assays. The real-time PCR : method has a very large dynamic range of starting target molecule : determination (at least five orders of magnitude). Real-time quantitative : PCR is extremely accurate and less labor-intensive than current quantitative : PCR methods. 我想real time pcr是经过label的sequence在作PCR时会放出萤光 可藉发光的程度知道pcr有没有作成功 量有没有被放大 照样产物就不会被污染了 也比较快 传统的方法是做完後要跑gel 那rt-pcr是用来作RNA的 先经过reverse transcriptase转成cDNA 再去做PCR -- Winners never quit quitters never Win --

※ 发信站: 批踢踢实业坊(ptt.csie.ntu.edu.tw) ◆ From: 140.112.226.21