课程名称︰生化 课程性质︰必修 课程教师︰吴世雄 开课学院：理学院 开课系所︰化学系 考试日期（年月日）︰2011/5/27 考试时限（分钟）：2小时 是否需发放奖励金：是 （如未明确表示，则不予发放） part 1 single choice 1. which of the following is true about the ABO blood groups? a. all 3 of the blood groups have an α-L fucose group attached b. type O blood is the universal donor because it has an α-L fucose group c. type B blood has a β-N-acetylgalactosamine group d. type AB blood is the universal donor 2.chitin, which forms the exoskeletons of insects, is composed of a. α(1->4)linked N-acetylglucosamine resudues b. β(1->4)linked N-acetylglucosamine resudues c. α(1->4)linked glucose residues d. β(1->4)linked glucose residues 3.a bacterial cell wall is composed of: a. a polysaccharide consisting of a single type of monosaccharide unit and cross-linking oligopeptides b. a polysaccharide consisting of 2 types of monosaccharide units and cross-linking oligopeptides c. a polysaccharide consisting of 3 types of monosaccharide units and cross-linking oligopeptides d. 2 different polysaccharide and 1 type of oligopeptide, which are cross-linked. 4.the human lipoproteins with the lowest density are a. chymomicrons b. HDL c. IDL d. LDL e. VLDL 5.which of the following isn't a function of cholestrol in the human body? a. precursor of steroid hormones b. energy storage compound c. precursor of bile salts d. component of cell membranes e. all of these are function of cholestrol in the human 6.the committed step in choleserol biosynthesis is catalyzed by a. HMG-CoA synthase b. HMG-CoA reductase c. mevalonate kinase d. squalene monooxygenase 7.the yield of ATP from the complete oxidation of glucose is lower in muscle and brain from that in kidney, liver, and heart because a. there are fewer mitochondria in muscle and brain cells b. muscle and brain cells have a lower requirement for ATP c. different shuttle mechanism operate to transfer electrons from the cytosol to the mitochondria in the sets of tissues d. none of the above 8.a characteristic of the glycerol phosphate shuttle is a. it shuttles NADH across the mitochondrial membrane to yield 5ATP/NADH b. it shuttles the electrons from NADH across the mitochondria membrane to FADH2, yielding 5ATP/NADH c. it only operates efficiently at high levels of NADH d. malate is a key component in the shuttle process 9.which of the following describes a use for acetyl-CoA as an important intermediate in metabolism ? a. breakdown to CO2 and water, yielding much energy. b. synthesis of terpenes and steroids c. synthesis of oxaloacetate in plants d. synthesis of fatty acid e. all of these are reasons why acetyl-CoA is a central molecule in metabolism 10. which of the following is true regarding the control of pyruvate dehydrogenase? a. it's inhibited by ATP b. it's inhibited by NAD+ c. it's activated by acetyl-CoA d. it's inhibited by succinyl-CoA e. none of these are true 11.the reaction in which malate is oxidized to oxaloacetate isn't thermodynamically favored. it takes place because a. it's coupled to ATP hydrolysis b. it involves substrate-level phosphorylation c. the product is continuously used up in the next reaction of the cycle, which is thermodynamically favored. d. it's coupled to a strong reduction 12.the vitamin thiamine is important in transferring all of these types of groups, except: a. 2-carbon sugar fragments b. 3-carbon sugar fragments c. 4-carbon sugar fragments d. sugar fragments which contain a carbonyl group(C=O) e. transfer all 13. the enzyme phosphopentose isomerase is characterized by all the following except a. it catalyzes the interconversion of ribose-5-phosphate and ribulous-5-phosphate b. there is no requirement for ATP c. it converts a ketone to an aldose d. it catalyzes an inversion of configuration at carbon-3 14. fatty acid synthase binds growing fatty acids a. by an ester linkage to a side chain serine b. by a thioester linkage to a side chain of cysteines c. as a Schiff base to a side chain of lysine d. by a thioester linkage to a phosphopantetheine linked to a serine 15. how do uncoupling agents affect the electron transport chain and oxidative phosphorylation? a. they block the flow of electrons, so proteins aren't pumped, and ATP synthesis ceases. b. they remove electrons from the chain, so less protons are pumped, and ATP synthesis decreases c. they block the flow of protons through the ATP synthase, so ATP synthesis ceases. electron flow and proton pumping are also halted d. they provide an alternative path for protons to re-enter the mitochondrial matrix, so ATP synthesis decreases. electron flow and proton pumping are not affected 16.which of the following isn't a component of the pyruvate dehydrogenase complex? a. pyruvate dehydrogenase b. dihydrolipoyl transacetylase c. dihydrolipoyl dehydrogenase d. pyruvate dehydrogenase kinase e. aconitase 17. which of the following statements concerning the anabolism and catabolism of fatty acids is true? a. biotin is required for oxidation of fatty acids but not for anabolism. b. NADPH is required for anabolism of fatty acids and not for catabolism c. anabolism occurs in the intermembrane space of mitochondria while catabolism takes place in the mitochondrial matrix d. Acetyl-CoA isn't required for anabolism 18. which of the following are located in the inner mitochondrial membrane? a. all the components of the citric acid cycle and the electron transport chain b. all the components of the citric acid cycle but none of the components of the electron transport chain c. all the components of the electron transport chain but none of the components of the citric acid cycle d. all the components of the electron transport chain and one of the components of the citric acid cycle, namely the succinate dehydrogenase complex 19.if you are running away from a bear, a. both your liver cells and my leg muscle cells will be running glycolysis. b. both your liver cells and my leg muscle cells will be running gluconeogenesis c. your liver cells will be running gluconeogenesis and your leg muscle cells will be running glycolysis. d. your liver cells will be running glycolysis and your leg muscle cells will be running gluconeogenesis. 20. which of the following isn't true concerning control of pyruvate kinase? a. the phosphorylated form is less active b. it's inhibited by ATP c. it's activated by fructose 1,6-bisphosphate d. it's inhibited by low blood glucose levels e. all of these 21. which of the following lipids isn't found in biological membranes? a. triacylglycerols b. phosphoacylglycerols c. glycolipids d. cholestrol e. sphingolipids 22. how do the membranes of plant and animal cells compare? a. animal cell membranes tend to be more rigid than those of plant cells. b. animal cell membranes tend to be more fluid than those of plant cells. c. animal cell membranes tend to be more asymmetric than those of plant cells. d. animal cell membranes tend to be more symmetric than those of plant cells. 23.which statements are consistent with the fluid-mosaic model of membrane? a. all membrane proteins are bound to the interior of the membrane b. both proteins and lipids undergo transverse (flip-flop) diffusion from the inside to the outside of the membrane c. some proteins and lipids undergo lateral diffusion along the inner or outer surface of the membrane d. carbohydrates are non-covalently bonded to the outside of the membrane e. the term mosaic refer to the arrangement of lipids alone part 2 1. correlate the following names or terms in (A) with those in (B) A. a. biotin ;b.carnitine ;c.HMG-CoA synthetase ;d.NADPH ;e.PEP carboxylase f.glutathione ;g. phospholipase A2 ;h. fluoroacetate ;i.pyruvate carboxylase j. kinase ;k.NADH ;l.dinitrophenol ;m.cytochrome a ;n.ribulose-1,5-bisphosphate o.arachidonic acid;p.glucosamine sulfate ;q.pheophytin ;r.glycolate;s.squalene epoxide t. glucose-6-phosphate dehydrogenase ;u.vitamin E ;v.carbon dioxide w. hemoglobin ;x. lipase y.bile acid ;z. lovastatin B. 1. which cofactor involved in pyruvate dehydrogenase complex? k 2. in the pentose phosphate pathway of red blood cell (RBC), which compound should be kept in reduced form by NADPH for preventing the hemolysis of RBC?f 3.what is the inhibitor of HMG-CoA reductase z 4.what kind of enzyme or protein is abundant in snake venom g 5. which enzyme is the first enzyme in the PPP t 6. in mammalian animals, which enzyme is responsible for generating oxaloacete(OAA) for anplerotic reaction of TCA cycle?i 7. a very strong inhibitor of TCA cycle h 8. which molecules have cholesterol as a precursor?y 9. which compound is sold as an over-the counter drug used to help repair damaged cartilagep 10. the precursor of prostaglandins and leucotrientso 2.answer the following question briefly 1. both cellulose and α-amylose consist of(1->4)linked D-glucose units and can be extensively hydrated. Despite this similarity, a person on a diet consisting predominantly of α-amylose(starch)will gain weight, whereas a person on a diet of cellulose will starve why? 2.a "pulse-phase" experiment consists of incubating a small amount of 14C-labeled substance with the yeast extract just long enough for each intermediate in the pathway to become labeled (a) if [1-14C] glucose (glucose labeled at C-1 with 14C) is used as a substrate, what is the location of 14C in the product ethanol? (b) where would 14C have to be located in the starting glucose molecule in order to assure that all the 14C activity were liberated as 14CO2 during fermentation to ethanol? 3-5 见图 http://dl.dropbox.com/u/19163210/biochemistry/biochemtest.pdf 6. (a) what important enzymes involved the glyxylate cycle? Isocitrate Lyase and Malate Synthase (b) what is the main purpose of the cycle in germinating seed? 7. could you explain why the structure of glycogen is highly branched? 8. how many ATP equivalents will be generated when (a) pyruvate (b) succinate are completely oxdized to H2O and CO2? (show the number) 9. what is metabolic water, which is improtant for camel to live in desert? 10. what are ketone bodies ? what physiological conditions can generate ketone bodies? 11.carnitine is involved in fatty acid beta-oxidation. what role does carnitine play in fatty acid catabolism? 12. what is the function of aspirin Aspirin can inhibit the cyclooxygenase of the prostaglandin endoperoxide synthase 13.which amino acid of prothrombin will be modified by vitamin K-involving reaction for blood clotting?Glutamate 14.in glycolysis, which enzyme cleaves the C-C bond?aldolase 15.in TCA cycle, which intermediates are chiral compounds? 16.what are the advantages of compact arrangement of a multienzyme complex such as pyruvate dehydrogenase? ※ 编辑: jasonfghx 来自: 140.112.115.226 (05/29 10:54)