课程名称︰生物分子工程导论 课程性质︰选修 课程教师︰吴嘉文 开课学院： 开课系所︰化工系 考试日期（年月日）︰99/06/08 考试时限（分钟）：120分 是否需发放奖励金：是 （如未明确表示，则不予发放） 试题 : Ι.Choice (75%, 2.5% for each) 1.The modern discovery which disrupted the original Central Dogma is that the flow of genetic information is: a.Replication translation transcription b.Replication trasncription translation c.Transcription translation replication d.Transcription replication translation e.Translation transcription replication 2.What is the requirement for a template strand in DNA replication? a.it serves as a guide in determining the next nucleotide to be added according to the Watson-Crick base pairing scheme b.it serves as the start point for the new DNA strand c.it allows the DNA polymerase to move along it easily d.it is a substrate for the 3'-5' exonuclease activity 3.All the following describe the general mechanism of DNA synthesis, except: a.One strand is made 5'→3' while the other strand is made 3'→5'. b.The strands become separated during synthesis. c.Synthesis occurs in both directions from the starting site of synthesis. d.Synthesis of DNA is a very accurate process. e.All of these are correct. 4.Which of the following best describes the process of DNA replication in a prokaryote? a.Replication begins at a unique site and proceeds in one direction all the way around a typically circular chromosome. b.Replication begins at a unique site and proceeds in two directions, meeting about halfway around a typically circular chromosome. c.Replication begins at multiple sites, spreading outward until the entire typically circular chromosome is replicated. d.Replication begins at multiple sites, spreading outward until the entire typically linear chromosome is replicated. 5.The universal features of DNA replication include all the following, except: a.Release of PPi from a nucleoside triphosphate. b.Synthesis from the 5' end to the 3' end. c.Base pairing of A to T and G to C. d.Use of a primer. e.All of these describe DNA synthesis. 6.Having the ability to degrade DNA allows DNA polymerases to perform their job properly and efficiently. a.True b.False 7.The primer for in vivo DNA replication is: a.The 3' hydroxyl of the preceding Okazaki fragment. b.A short piece of RNA. c.A nick made in the DNA template. d.A primer is not always required for DNA replication. e.All of these are true. 8.Which of the following is not a function of DNA polymeraseΙ from E. coli? a.adding nucleotides to the primer strand b.3'→5' exonuclease activity c.5'→3' exonuclease activity d.proofreading 9.E. coli replication on the lagging strand a.is carried out by DNA polymeraseΙ b.is initially synthesized as Okazaki fragments c.is synthesized continuously d.had this DNA strand synthesized in a 3'-5' direction 10.Which of the following activities does E. coli DNA polymeraseⅢ lack? a.5'→3' polymerase b.5'→3' exonuclease c.3'→5' exonuclease d.E. coli DNA polymeraseⅢ has ALL of the above activities. 11.Transcription of RNA and replication of DNA are similar in all these ways, except: a.Nucleoside triphosphates are the precursors. b.Both strands of DNA are copied. c.Base pairing is used to copy the sequence in a precise manner. d.The chain grows for the 5' to the 3' end. e.All of these are similarities between RNA and DNA synthesis. 12.The universal features of RNA transcription include all the following, except: a.Release of PPi from a nucleoside triphosphate. b.Synthesis from the 5' end to the 3' end. c.Base pairing of A to U and G to C. d.Use of a primer. e.All of these describe RNA synthesis. 13.The sigma subunit of RNA Polymerase has all the following properties, except: a.It tells the RNA Polymerase where to sit down. b.It helps point the RNA Polymerase in the proper direction. c.It cause the RNA Polymerase to bind tightly to the DNA. d.It stays with the RNA Polymerase throughout synthesis. e.All of these describe the sigma factor. 14.These terms can be used interchangeably to denote the complementary strand in DNA, except: a.Template strand b.Coding strand c.Antisense strand d.Negative or "-" strand e.All these terms describe the complementary DNA strand 15.Chain termination occurs, in vivo, when: a.RNA Pol gets to the end of the DNA. b.The factor called rho(ρ) binds to the DNA. c.A hairpin loop forms in the template. d.Either a hairpin loop forms or rho is involved. e.All of these. 16.Which of the following correcting describes a difference between RNA & DNA polymerases? a.RNA polymerases usually do not need a template, while DNA polymerases do. b.DNA polymerases usually require a primer(i.e., they can only continue a strand, not start one), while most RNA polymerases do not. c.RNA polymerases usually synthesize introns, while DNA polymerases synthesize cistrons. d.RNA polymerases polymerize 5'→3', while DNA polymerases polymerize 3'→5'. 17.Which of the following offers the best description of a Pribnow box? a.A promoter consensus sequence located at approximately -35. b.A promoter consensus sequence located at approximately -10. c.A sequence forming a hairpin loop signaling the termination of transcription. d.A sequence immediately surrounding the start site of transcription. 18.The promoter region is one term use to describe where RNA Pol binds to DNA. a.True b.False 19.The DNA must form an open promoter complex before RNA Pol can bind to it. a.True b.False 20.The weakness of A-U base pairs at the end of the RNA molecule may help in dissociation of the new RNA product. a.True b.False 21.The majority of protein synthesis occurs in the a.nucleus b.mitochondrion c.cytoplasm d.nucleolus 22.As in RNA and DNA synthesis, the synthesis of proteins follows the following steps, except: a.Activation of monomerss b.Initiation c.Elongation d.Termination e.All of these are involved in all polymer synthesis 23.The following terms describe the genetic code, except: a.Triplet b.Non-overlapping c.Commas are used d.Degenerate e.All of these describe the genetic code. 24.Degeneracy allows all of the following efficiencies in the genetic code, except: a.More than one amino acid can bind to a tRNA. b.Each tRNA can bind to more than one codon. c.Most codons can bind to more than one tRNA. d.Fewer tRNA molecules are needed. e.All of these are true. 25.All 64 of the codons code for an amino acid. a.True b.False 26.The wobble base is the 3' letter of the triplet codon. a.True b.False 27.Which amino acids have unique codons? a.met, gly b.trp, met c.tyr, met d.stop, his 28.A tRNA was determined to have the following anticodon sequence: 3'-GAI-5' (I represents the base hypoxanthine). Indicate which of the following codons can form base pairs with this anticodon a.5'-CUA-3' b.5'-CUC-3' c.5'-CUU-3' d.all of the above 29.The genetic code is said to be degenerate. This means that a.each codon codes for more than one amino acid b.each anticodon can interact with many different triplet sequences in the mRNA, which may differ in any or all of three nucleotides c.many of the amino acids are coded for by different codons d.the code is universally used by virtually all species 30.The process of amino acid activation a.involves the formation of a peptide bond between the amino acid and tRNA b.takes place by formation of a mixed anhydride of the amino acid and tRNA c.involves the formation of a ester bond between the amino acid and tRNA d.none of the above Ⅱ.Questions (10%, 5% for each) 1.Describe all the necessary processes to get DNA from E. coli. 2.Describe the filter-binding assay and how to use this method to determine the relationships between codons and corresponding amino acids. Ⅲ.Explain the following items (15%, 2.5% for each) 1.Sticky ends 2.RNAi 3.Gel electrophoresis 4.Northern blotting 5.FISH(Fluorescence in-situ hybridization) 6.EDTA --

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