课程名称︰分子生物学 课程性质︰必修 课程教师︰周子宾 开课学院：生命科学院 开课系所︰生命科学系 考试日期（年月日）︰2008.12.18 考试时限（分钟）：100分钟 是否需发放奖励金：否 （如未明确表示，则不予发放） 试题 : A: Term Explanation(2% of each), 30% 1. Glycosylases 2. Excinuclease 3. XRCC1 4. Topoisomerase 5. Photolyase 6. Chiasmata 7. Fts ring 8. Tus and ter site 9. Chi site 10.F-plasmid 11.AP endonuclease 12. iDNA 13.Licensing factor 14.Synaptonemal complex 15. Episome B: Assay choice 10 (8% of each), 80% (a) Describe how to control COlE1 plasmid replication. (b) How to determine unidirectional replication? (c) How to create the replication fork at an replication origin C of E.coli? (d) How the Uvr repair system fuctioning in DNA excision repair? (e) DNA assimilation involves with (1)chi site (2)Rec complexes (3)Ruv factors. please describe the mechanism. (f) What are the "SOS response" and "Error-prone repair by DNA polymerase V"? and how the RecA to involve with it? (g) Describe mutT,M,Y system handles the consequences of oxidative damage. (h) Describe θform, D-loop, and rolling circular in circular DNA replications and how to copy the end of DNA in a linear DNA for replication. (i) How yeast to switch silent and active loci for mating type? (j) Describe the conserved NHEJ mechanism for repairing in eukaryotic cells. (k) Describe the 6 vir loci in T-DNA transfer. (l) Describe the relation of licensing factor,ARS,ORC,Cdc6,and MSM of yeast. --

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