课程名称︰ 分析化学乙下 课程性质︰ 系所必修 课程教师︰ 刘春樱 开课学院： 理学院 开课系所︰ 化学系 考试日期（年月日）︰ 2008/4/23 考试时限（分钟）： 120 min 是否需发放奖励金： 是 （如未明确表示，则不予发放） 试题 : 1. Define the following terms: (24%) a) stray light b) matrix modifier c) photodiode array spectrophotometer d) hollow cathode lamp e) flow injection analysis e) chemiluminescence 2. What are the advantages and disadventages of decreasing monochromator slit width? (3%) 3. Describe two mechanisms broading the lines to 10^-3 to 10^-2 nm in atomic spectroscopy. (6%) 4. Explain how the background correction techniques work: (a) beam chopping, (b) Zeeman (8%) 5. Explain what is meant by spctral, chemical, ionization interference. (9%) 6. How to get the composition of a complex with the method of continuous variation? (10%) 7. Explain how a laser generates light. List improtant properties of laser light. (10%) 8. Describe the differences between the following and list any particular advantages possessed by one over the other: (18%) a) phototubes and photomultipier tubes b) conventional and diode-array spectrophotometers c) fluorescence immunoassay and time-resolved fluorescence immunoassay. 9. (a) What is aptamer? (b) To measure the protein immunoglobulin, an aptamer is seleted that binds IgE. Describe how "ruthenium complex with a flat aromatic structure" can be used as "molecular light switch." (10%) 10. Describe the principle for the luminescent determination of intracellular O2 concentration with 4,7-diphenyl-1,10-phenanthroline Ru(II) complex. (10%) --

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