作者jillrong (Grace)
站内NHRI_NTHU
标题[问题] 彭老师12/31上课 投影片的问题
时间Mon Jan 4 20:24:18 2010
在第53页 倒数第二行
HAT is a common selection medium and only hybridoma can survive and grow
而54页第一点
HAT medium contains hypoxanthine, aminopterin, and thymidine
同页第四点
Two key enzymes in these nucleotide salvage pathways are thymidine
kinase (TK) and hypoxanthine-guanine phosphoribosyl transferase
(HGPRT)
第五点又说
Cells produce these enzyme can grow on HAT medium, which supplies
a salvageable purine and thymidine
↑这是说TK和HGPRT这两种酵素可使cell成长在HAT medium中吗?
如果是的话,那麽,最後一点为什麽会说
Normal cells can grow in the HAT medium, but TK- and HGPRT- cell
cannot
有人可以告诉我吗? 谢谢!
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1F:→ jillrong:喔 耍笨了 没看到最後一行各有小小的 minus符号 01/04 20:31
2F:→ jillrong:看到後一页slide才发现... 01/04 20:33