如题 https://www.youtube.com/watch?v=x5yPkxCLads 写报告之余唱唱歌 XD (请自行忽略打广告的部分) ====== The PCR Song ====== There was a time when to amplify DNA, 有段时间需要大量复制某段DNA的时候 You had to grow tons and tons of tiny cells. 你必须培养成千上万个小细胞 Then along came a guy named Dr. Kary Mullis, 之後出现一位名叫凯瑞慕理斯博士的家伙 Said you can amplify in vitro just as well. 他说你也可以进行细胞外的大量复制 Just mix your template with a buffer and some primers, 只要DNA模版、缓冲液跟起始端混合 Nucleotides and polymerases, too. 还要加上核酸跟聚合酉每 Denaturing, annealing, and extending. 变性、黏合及延伸 Well it's amazing what heating and cooling and heating will do. 加热、冷却、再加热的结果让人惊异 PCR, when you need to detect mutations. PCR，当你需要侦测突变的位置时 PCR, when you need to recombine. PCR，当你需要进行基因重组时 PCR, when you need to find out who the daddy is. PCR，当你需要知道谁是孩子的爹时， PCR, when you need to solve a crime. PCR，当你需要知道谁是凶手时。。。 --

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