作者ggleon (喜)
看板NCKU_MI99
标题Re: 今天的分子生物
时间Sat Sep 20 14:58:02 2008
※ 引述《cyping ( )》之铭言：
: Homework
: 1) Retrieve a full-length cDNA coding for at least 2 domains from database
: (with accession number);
: show the DNA and protein sequences and the domain regions;
: predict motifs;
: design primers (forward and reverse) for cloning into pEGFP-C1 and pEGFP-N1
: vector (2-3 pages)
: 2) Read one of the assigned articles
: (Science 321:1499, 2008; Trends Microbiol 16:414, 2008).
: Write a one-page research proposal in the following order:
: 1)background/significance,
: 2) hypothesis,
: 3) specific aims/anticipated results,
: 4) impact. Do not copy and paste.
: 3) Homework due on September 30, 2008, 5 pm. English only.
: Please submit a hard copy to my lab (x5592).
: Attn: Ninthin or Esti. Also, email to: [email protected]
: part 1
: 可以从网站上找到 full-length cDNA(需标明accession number)
: 并标明 DNA domain 与 protein domain motifs
: 设计primer
: part 2
: 老师给了两篇paper
: science 321:1499,2008
: http://www.sciencemag.org/cgi/reprint/321/5895/1499.pdf
: Trends Microbiol 16:414,2008
: Thermoacidophilic proteins for biofuel production
: 要挑一篇写
: 缴交时间 2008/9/30 pm 5:00 English only
: 大家加油 赶快写一写吧 ^^
http://www.ncbi.nlm.nih.gov/Structure/lexington/lexington.cgi?cmd=rps
输入蛋白质序列即可
应该可以看到Domain.....
询问之前大学教生资的老师
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