1.Acid phosphatase is glycoprotein. Cytochemical location of this enzyme could be approached by traditional enzyme cytochemical methods and immunocytochemical method. If you are a professor, you are going to submit a proposal to financial-support organization. How cam you do in systematic approaches? 2.Cryobiology is a recently well-developed integrated field in biological and physical sciences. Would you please mention, principle, method, application and limitations of different cytotechniques, e. q.. cryopreservation of sperm and/or plant calli, freeze-drying, freeze-substitution, freeze-fracture, freeze-etching and cryoSEM. 3.In the lecture of Botanical Electron Microscopy, we have discussed a lot about three dimensional images of macromolecule and tissue. Could you correlate their instrumentation, method and application. 4.gene expression of eukaryotic cells are reflected in forms of RNA(hnRNA) whose molecular weight is much higher than that of mRNA. biochemical study of hnRNA slicing is processed by the participation of small nuclear RNA. Dr. Chow and her adviser approached RNA splicing with electron microscopic technique and cell fractionation. Could you figure out a general scheme of their study. 5.Dr. Monday's project proposal on status of Oil Dropletes in Dry Seed of Zinnia elegans. Now, you are a member of panel reviewer and your should insist what are the minimal requisite of complementary studies. 6.Point out the importance, application and data interpretation of the following techniques, such as negative staining, shadowing, and immunolabelling, etc.,for Molecular and Cell Biology. 7.artifacts and troubles are often found in electron microscope and the processing of thin section. Could you figure out them in detail and present the criteria and/or troubleshooting to correct them. 8.compose a solid and meaning paragraph containing the following terns and techniques without missing any one: 1. Molecular structure 2. TEM 3. SEM 4. Cyrotechniques 5. NMR 6. X-ray diffraction 7. STM 8. Electron diffraction 9. AFM 10. Ultrastructure 这份报告 要在期末考周礼拜二电显课交给老师 --

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