作者zinga (zinga)
看板Chemistry
标题生化问题
时间Sat Oct 21 16:09:31 2006
关於negative cooperativity一般印象是四级结构有
不过书上提到两种情况发生在三级结构也很常见 恳请板上大大解释一下原因
(1)The protein is a single polypeptide with two lignd-binding sites,each
having a different affinity for the ligand.
(2)The protein is a single polypeptide with a single ligand-binding site.
As purified,
the protein preparation is heterogeneous,containing some
protein molecules that are partially denatured and thus have a lower
binding affinity for the ligand.
顺便一问 上述黄底字是甚麽意思??
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1F:推 arlix:不是纯的native enz....有些在纯化过程中会不小心denature 10/21 22:22