qPCR NEWS June 2008 - Update in RNA integrity Dear researcher, dear Gene Quantification page reader, Our newsletter informs about the latest news in quantitative real-time PCR (qPCR and qRT-PCR), which are compiled and summarised on the Gene Quantification homepage. The focus of this newsletter issue is: - RNA integrity page updated! - CAmpER - Real-time PCR analysis software - Event calendar 2008 - qPCR meetings 2008 - qPCR application workshops ---------------------------------------------------------------------------= ----- If this newsletter is not displayed correctly by your email client, please use following link: http://qPCRnews.gene-quantification.info/ ---------------------------------------------------------------------------= ----- RNA Quality control The assessment of RNA integrity is a critical =EF=AC=81= rst step in obtaining meaningful gene expression data. Working with low- quality RNA may strongly compromise the experimental results of downstream applications which are often labour-intensive, time- consuming, and highly expensive. Using intact and integer RNA is a key element for the successful application of modern molecular biological methods, like qRT-PCR or micro-array analysis. To verify RNA quality nowadays commercially available automated capillary-electrophoresis systems are available which are on the way to become the standard in RNA quality assessment. Pro=EF=AC=81les generated yield information on RNA concentration, allow a visual inspection of RNA integrity, and generate approximated ratios between the mass of ribosomal sub-units. More info here: http://RNA-integrity.gene-quantification.info/ ---------------------------------------------------------------------------= ----- Updated publications - RNA Quality control Impact of RNA quality on reference gene expression stability. Claudina Angela P=C3=A9rez-Novo, Cindy Claeys, Frank Speleman, Paul Van Cauwenberge, Claus Bachert, and Jo Vandesompele BioTechniques 2005 Volume 39, Number 1: pp 52-56 Improved RNA quality and TaqMan=C2=AE Pre-amplification method (PreAmp) to enhance expression analysis from formalin fixed paraffin embedded (FFPE) materials. Li J, Smyth P, Cahill S, Denning K, Flavin R, Aherne S, Pirotta M, Guenther SM, O'Leary JJ, Sheils O. BMC Biotechnol. 2008 8:10. Optimization of the PAXgene blood RNA extraction system for gene expression analysis of clinical samples. Chai V, Vassilakos A, Lee Y, Wright JA, Young AH. J Clin Lab Anal. 2005;19(5): 182-188. Successful downstream application of the Paxgene Blood RNA system from small blood samples in paediatric patients for quantitative PCR analysis. Carrol ED, Salway F, Pepper SD, Saunders E, Mankhambo LA, Ollier WE, Hart CA, Day P. BMC Immunol. 2007 8:20. Impact of RNA degradation on gene expression profiles: assessment of different methods to reliably determine RNA quality. Copois V, Bibeau F, Bascoul-Mollevi C, Salvetat N, Chalbos P, Bareil C, Candeil L, Fraslon C, Conseiller E, Granci V, Mazi=C3=A8re P, Kramar A, Ychou M, Pau B, Martineau P, Molina F, Del Rio M. J Biotechnol. 2007 127(4): 549-559. REVIEW: RNA integrity and the effect on the real-time qRT-PCR performance. Simone Fleige & Michael W. Pfaffl Molecular Aspects of Medicine 27 (2006) 126=E2=80=93139 Successful RNA extraction from various human postmortem tissues. Heinrich M, Matt K, Lutz-Bonengel S, Schmidt U. Int J Legal Med. 2007 121(2): 136-142. RNA quality in frozen breast cancer samples and the influence on gene expression analysis =E2=80=93 a comparison of three evaluation methods usin= g microcapillary electrophoresis traces. Strand C, Enell J, Hedenfalk I, Fern=C3=B6 M. BMC Mol Biol. 2007 8: 38. Preanalytical mRNA stabilization of whole bone marrow samples. Langebrake C, G=C3=BCnther K, Lauber J, Reinhardt D. Clin Chem. 2007 53(4): 587-593. ---------------------------------------------------------------------------= ----- With the new qPCR INFO PORTAL and all the presented tools we will help you with to find the right information about qPCR and related topics in Molecular Biology in the literature and in the World Wide Web. =3D> Papers / Protocols / Methods / Databases / Alets / Feeds / Books / Forums / E-mail / Directory http://infoportal.gene-quantification.info/ ---------------------------------------------------------------------------= ----- CAmpER - Real-time PCR analysis software CAmpER - Calculation of Amplification Efficiencies for RT-PCR experiments is a tool for the automatic analysis of real time PCR experiments. http://www.gene-quantification.de/download.html#camper Automatic analysis, annotation and storage of real-time PCR experiments performed with different real-time PCR systems, currently the LightCycler=C2=AE and the Opticon=C2=AE. ---------------------------------------------------------------------------= ----- Upcoming Events World-wide academic and commercial qPCR Events http://events.gene-quantification.info/ Symposia, Meetings, Conferences, Workshops, Seminars, Online-Seminars, qPCR Education Program, ...etc.. Please submit your qPCR event here =3D> events@gene- quantification.info ---------------------------------------------------------------------------= ----- qPCR SYMPOSIUM BENELUX The prominent and still growing place taken by real-time quantitative PCR in applied and fundamental research and clinical diagnostics almost appears obvious. However, it is clear that contributions made by various scientists and companies in the field during the last decade rendered this technology useful and affordable for many users. More info =3D> http://www.gene-quantification.de/meetings.html#benelux Importantly, the qPCR domain is still in constant evolution, making it sometimes hard to stay informed about new methodological approaches or original studies using the real-time PCR. Therefore, we have scheduled a one day "Benelux qPCR Symposium" on October 6th 2008, giving the opportunity to the scientific community to get informed and discuss various aspects of real-time PCR (including but not limited to new applications, assay optimization and validation, new technologies, etc.). Scientific talks, posters sessions and industrial booths will be at the menu. Download poster =3D> http://www.gene-quantification.de/qpcr_benelux_poster= ..pdf ---------------------------------------------------------------------------= ----- qPCR Symposium USA 10. - 13. November 2008 Clarion Hotel San Francisco Airport, Millbrae, CA , USA More info =3D> http://www.gene-quantification.de/meetings.html#qpcr_usa - High throughput platforms: High throughput applications, real-time RT-PCR arrays, digital PCR - Forthcoming technologies: Immuno PCR, Methylation sensitive PCR, SNP analysis, High resolution melt, microRNA detection, - Multiplex technologies - Single-cell qPCR: Pre-amplification techniques, sub-cellular PCR, Expression heterogeneity, laser microdissection, FACS sorting, Enrichment of rare cells - Multimarker diagnostics: Disease markers, Tissue specific markers, Cancer markers, Stem cells, Differentiation markers, Cancer stem cells - Real-time PCR Expression Profiling: multivariate and multiway expression profiling, temporal expression profiling, spatiotemporal maps - Pre-analytical Steps: Sampling technologies, Extraction methods, Reverse Transcription, Quality Control, Standards, Standard Operating Procedures, Interlaboratory Exercises - Normalization & Standardization: Normalization strategies, Reference genes, Spikes, Standard curves, multiplexing, inter-run calibrators, quantification strategies, mRNA degradation - Data management and data treatment: software applications, data mining, data visualization, biostatistics, multivariate statistics ---------------------------------------------------------------------------= ----- qPCR WORKSHOP TATAA Biocenter Germany - qPCR Application workshops At the TATAA Biocenter Germany we offer qPCR application workshops, the 3-day Core Module and a 2-day Biostatistics Module. qPCR courses are held in regularly in G=C3=B6teborg, Sweden, in English and in Freising- Weihenstephan, Germany, in German and English, and in Prague, Czech Republic in English and Czech. Depending on the occasion the workshop language and the different prices may apply. Further customized workshops and specialized trainings will be held as well across Europe and world-wide. TATAA Biocenter Germany courses are held in cooperation with the Institute of Physiology, located at the Technical University of Munich, in Freising-Weihenstephan, near Munich, very close to the Munich Airport (MUC). For more information and to register for the qPCR application workshops, please see our web page: http://tataa.gene-quantification.info/ Course Occasions 2008: 3-day qPCR Core Module (Mon. - Wed.) and 2-day BioStatistics Module (Thu. - Fri.) * 7 - 11th July 2008 (in Freising, Germany, English language) * 15 - 19th September 2008 (in Freising, Germany, English language) * 13 - 17 October 2008 (in Freising, Germany, Kurs wird in DEUTSCH gehalten, German language) * 24 - 28th November 2008 (in Freising, Germany, English language) Please register here =3D> http://www.tataa.com/Courses/Courses.html ---------------------------------------------------------------------------= ----- Forward Please send the qPCR NEWS to further scientists and friends who are interested in qPCR ! Best regards, Michael W. Pfaffl responsible Editor of the Gene Quantification Pages http://www.gene-quantification.info ---------------------------------------------------------------------------= ----- If this newsletter is not displayed correctly by your email client, please use following link: http://qpcrnews.gene-quantification.info/ The qPCR NEWS and the Gene Quantification Pages are educational sites with the only purpose of facilitating access to qPCR related information on the internet. The qPCR NEWS and the Gene Quantification Pages are edited by Michael W. Pfaffl and powered by BioScience Events. Copyright =C2=A9 2005 - 2008 All rights reserved. Any unauthorized use, reproduction, or transfer of this message or its contents, in any medium, is strictly prohibited. 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