※ 引述《[email protected] (拉拉:您好 掰掰)》之铭言： > 这是题目 > Q:The size of Okazaki fragments differs in different organisms. > What might influence this size? How might you test this possibility? > 这是找的答案 我有努力翻过,但是还是看的哩哩落落/_\ 真的是无计可施 > 所以来请教各位大大,字数很多 真对不住~ 不用全翻 只跪求重点<(_ _)> > Ans: > The size of Okazaki fragments in a given organism reflects the specific > kinetics governing the association between primase and the other replication > components during lagging strand synthesis in the cell. Primase only joins > the replication fork periodically, sticking around just long enough to > synthesize an RNA primer and thereby trigger lagging strand synthesis. > The frequency with which primase joins the fork can differ between organisms, > leading to characteristic differences in the length of the Okazaki fragments. > In organisms where primase has a high affinity for the other replication > factors (such as helicase), for example, the enzyme will associate with the > fork more frequently, resulting in shorter Okazaki fragments. Alternatively, > when the primase has less affinity for the fork, it will associate less > frequently, leading to longer Okazaki fragments. 冈崎片段的长度与复制叉对primase吸引力成反比， 吸引力强代表primase在复制叉附近的consentration高 所以okazaki fragments会比较短 > You could experimentally address the role of primase and its association with > the replication fork in determining Okazaki fragment length by altering the > association and seeing what effect this has on fragment length. For example, > you could increase or decrease the concentration of primase in a cell to see > if higher concentrations (which should lead to more frequent primase-fork > interactions) result in shorter Okazaki fragments, and if lower concentrations > lead to longer ones. You could also isolate mutant forms of primase that have > higher or lower affinities for helicase (or other replication components) and > ask whether the Okazaki fragment length is altered in the mutant cells. 控制primase之consentration，观察okazaki fragment 控制法有1.增加primase浓度 2.增加helicase与primase之亲合力 请指正^^" -- ┌─────◆ＫＫＣＩＴＹ◆─────┐KKMAN团队 全新力作 ◎◎KKBOX◎◎ │ bbs.kkcity.com.tw │知名歌手通通都有 所有新歌想听就听 └──《From:140.112.249.232 》──┘※※ 内容丰富多元的线上音乐台 ※※ --