由於9n年大考出了一题,结果与旧教材及每个学过高中生物的学生所认识不同 我查了好久, 而国内大学对这类简单的化学分析却没有提出答案, 任由考试引导教学内容 也不妥, 至少我看到的报告是 大陆--滤纸效果--胡黄ab 香港--滤纸效果--胡黄ab 美国--高中教材参考--滤纸效果--胡黄ab 其他网页--薄层分析效果--胡ab黄 只有最後一项与该年大考所示答案一致, 而解释却共用了极性问题来概括 困扰了两年, 在google用不同词组搜寻, 看到这讨论..摘述部份内容 "We do a chromatographic separation that sounds very much like Michael's and I thought the pigment order was chlorophyll b chlorophyll a xanthophyll 2 xanthophyll 1 (gray, "ides") and carotene at the solvent front. ... If run on a silica gel plate, I would expect the following pigments: carotenoids at solvent front, followed by a dull grey band of chlorophyll decomposition products (depending on how old the spinach prep is), followed by chlorophyll a then b then three xanthophylls (one fairly bright with the others fairly dull). ... We also do a separation of spinach in petroleum ether/ acetone on paper. The separation is based on the polarity of the pigments - least polar should migrate farthest. So at/ near the solvent front is carotene followed by xanthophyll, chl a and chl b. This order is the same as most other replies to the list. Every once in a while we get odd results. Check all the components of your set up for contamination. " http://biae.clemson.edu/biolab/spinach.html 在其中似乎也有些argue, 但大概可以见到两分析方法有不同结果 因此现在都只会教同学记跑最快的胡萝卜素, 其他我没有answer 知道者其实只要将几个band跑吸光光谱就好了, 当年若是立即有各大学立刻讨论,也许不会有此困扰 还好没影响分数, 不然这帐要向谁算 -- Ξ Origin: 中兴大学天枢资讯网 <bbs.nchu.edu.tw> Ξ From : 203.68.192.30