ConA-agarose column (1x2cm) http://www.vectorlabs.com/products.asp?catID=211 Agarose Concanavalin A的简称, Concanavalin A是一种lectin, 会认alpha-linked man nose, 你的蛋白如果有被修饰过, 说不定可以用这个column来做affinity purification. phenyl Sepharose CL-4B column(1.6x15cm) 一种HIC(hydrophobic interaction chromatography)column, Amersham/GE所出. DEAE Sepharose CL-6B column(1x15cm) Amersham/GE 出的weak anion exchanger, DEAD是Diethylaminoethyl的简称, Sepharose 是Amersham注册的一种agarose. 另一种CM(Carboxymethyl)是weak cation exchanger. weak与strong跟结合力没关系, 那是描述不同pH范围内游离化程度的术语. 以上两种Amersham/GE的columns/bead, 在他们的管柱手册都有详细且实用的说明, 说实 在的, 以营利公司而言, 我目前为止还没看过写的比它好的. 对纯化蛋白质非常有帮助 在Amersham/GE的网站(太长自己接起来 http://www4.amershambiosciences.com/APTRIX/upp01077.nsf/ Content/orderonline_handbooks )里, 只要你注册过(免费), 就可以免费下载这些每本值约20 美金的手册, 这些手册名称如下 Handbooks: Product code Item Hardcopy Price 18-1142-75 Recombinant Protein Handbook: Protein Amplification and Simple Puri fication $20.00 add to cart 18-1157-58 GST Gene Fusion System Handbook $8.00 add to cart 18-1022-29 Affinity Chromatography Handbook: Principles and methods $20.00 ad d to cart 18-1037-46 Antibody Purification Handbook $20.00 add to cart 18-1132-29 Protein Purification, Handbook $20.00 add to cart 18-1022-18 Gel Filtration: Principles and methods $20.00 add to cart 11-0004-21 Ion Exchange Chromatography: Principles and methods $17.00 Product code Item (Download only) 80-6429-60 2D Electrophoresis using immobilized pH gradients - Principles and Methods 18-1134-16 Reversed Phase Chromatography: Principles and methods 18-1020-90 Hydrophobic Interaction Chromatography: Principles and methods 18-1009-07 Chromatofocusing with Pollybuffer and PBE 18-1124-26 Expanded Bed Adsorption: Principles and Methods 18-1140-62 Micro Carrier Cell Culture: Principle and methods 18-1152-69 Ficoll Paque Plus: isolation of lymphocytes 18-1115-69 Percoll Methodology and applications 80-6013-88 Protein Electrophoresis: 1D technical manual 18-1138-07 The Complete Guide to Protein Labelling and Detection 63-0035-28 Fluorescent Imaging: Principles and Methods 63-0048-49 Microoarray handbook 80-6343-34 Automated Staining of Polyacrylamide Gels with Processor Plus Proto col Guide for Silver and Coomassie Staining 80-6437-96 PCR product analysis using semi-dry flatbed gel electrophoresis 80-6447-27 Blot Processing with the Processor Plus Protocol Guide for Western Blotting -- ┌─────◆ＫＫＣＩＴＹ◆─────┐▇─┐ 优质连线服务隆/重/豋/场!! │ bbs.kkcity.com.tw │┴ └─▇ KKADSL 带你环游全世界 └──《From:143.48.8.154 》──┘ KKADSL ┴ http://adsl.kkcity.com.tw