看板Biology
标 题Re: DNA保存
发信站台大电机 Maxwell BBS (Thu Sep 11 11:26:29 2003)
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※ 引述《[email protected] (why ~.~)》之铭言:
: ※ 引述《simplewish (simplewish)》之铭言:
: : 一般实验室都把DNA保存在-20度冰箱吧
: : 不过为什麽需要保存在这样的温度
: : 不能直接放在室温下或是普通的冰箱里面??
: 不是应该放在摄氏-80度的冰柜吗?
For the short-term storage (up to one month), it is fine to keep DNA
(dissolved in TE buffer, pH 8.0) at 4 deg, centigrade. However, for the
long-term storage (say, more than six months), it is better to keep DNA
(dissolved in TE buffer, pH 8.0) at -70 (or -80) deg centigrade. In between,
it is all right to keep DNA at -20 deg centigrade.
--
"...What science has accomplished is to tell us that this happens because
organisms contain genes in them and that the future organism is written in
this 'somehow'. And 'somehow' is what we have to explain. We have to say not
'somehow', but 'how'. " --- Sydney Brenner
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