作者yiliang (yiliang)
看板BioChem_92
标题Re: 作业来讨论呗
时间Wed Sep 3 22:14:04 2003
※ 引述《angex (倾听湖水的涟漪)》之铭言:
: ※ 引述《stemcell ( 一尾 @~~~~)》之铭言:
: : 那total volume 是不是跟 void volume 大小一样阿
: 不一样喔
: void volume是所有gel颗粒的外部体积总和(不包括内部孔洞体积)
这是我查到的
Void Volume
The void volume is the volume of mobile phase (Vm or V0) in a column.
For example, if the stationary phase occupies 40% of the total column
volume, the void volume would be 60% of the total column volume.
Consider a column that is 25 cm long with an inner diameter of 1 cm.
The total column volume is 19.6 mL (V = p r2L = 3.14 * 0.52 * 25 cm).
If the mobile phase occupies 60% of the column volume, the void volume
is 11.8 mL.
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