: 图示里有两段奇怪的地方, 就是图2-set1 出现杂讯由高降低的变化. : 另一个就是注入 dNTPs 後 由图2-set2 约30分钟後 变成 图2-set3 的变化. 老师，次世代定序平台中，Roche 454 搭配的 nucleotide substrate 同样是 dNTPs. Roche 是我的 sponsor 所以之前这部份是有念过一些文献，比方说有国图帐号的可以 参考 DNA Polymerases: Discovery, Characterization and Functions in Cellular DNA Transactions (2010) 这本书第 61 页： 2.2 The DNA Polymerase Reaction (……) DNA pols incorporate a base by the following five-step-mechanism: first, a correct hydrogen bonding between the incoming dNTP and the tempting base is established (A to T and G to C and vice versa); second, water is excluded from the active site; third, the geometric selection in the active site takes place; fourth, the dNTP binding affinity leads to an induced conformational change in the active site and fifth, the phosphodiester bonds is formed between the last base of the primer and the new nucleotide by the release of pyrophosphate. 第 62 页有图示说明，搭配着看会比较容易理解这个反应过程。 没有国图帐号看不到图的话也没关系，Illumina 今年也有一篇 DNA polymerases drive DNA sequencing-by-synthesis technologies: both past and present，PMC 上可看到全文 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4068291/ Illumina 这 review 不只有类似的图片说明，APPLICATIONS OF DNA POLYMERASE FOR EMERGING SEQUENCING TECHNOLOGIES 那段的结尾： Besides the nanopore-based sequencing approach, a protein, transistor-based sequencing method, leveraging electrical conductance measurement of 29 DNA polymerase reactions has been reported (Chen et al., 2013b). Unfortunately, this study is currently called into question, and the merits of this particular method must be reevaluated (Chen et al., 2013b). 最後附上一篇 Duke 的 "室温" 实验报告— [Nano Lett. 2008] A DNA Nanotransport Device Powered by Polymerase 29 http://pubs.acs.org/doi/abs/10.1021/nl802294d 就是这样才对科技部袒护交大那非常勇健根本就外星来的聚合酶实验报告非常愤怒啊 --

※ 发信站: 批踢踢实业坊(ptt.cc), 来自: 180.218.166.23 ※ 文章网址: http://webptt.com/cn.aspx?n=bbs/AfterPhD/M.1416336410.A.ED1.html ※ 编辑: Shilia (180.218.166.23), 11/19/2014 02:50:09